This bill, titled the "America's Living Library Act," establishes a pilot program known as the America's Living Library Project . Its primary purpose is to facilitate the collection of new genomic data from various species, including animals, plants, fungi, and microbes, found within units of the National Park System. The Secretary of the Interior, acting through the Director of the United States Geological Survey, is tasked with overseeing this initiative. The program requires extensive interagency coordination, involving entities such as the National Park Service, the Department of Agriculture, the Smithsonian Museum of Natural History, and the National Institutes of Health. All collection activities must adhere to existing environmental protection laws, including the Endangered Species Act, Migratory Bird Treaty Act, and Marine Mammal Protection Act. An office within the USGS will administer the program, with authority to enter into contracts and receive in-kind contributions from biotechnology companies, subject to conflict-of-interest vetting. A core provision is the establishment of a publicly available genomic database to house the collected data, including long-read sequences, genome assemblies, and associated metadata. This database will integrate with existing taxonomic systems and will withhold sensitive information, such as precise collection locations, to protect resources. The bill emphasizes the importance of leveraging existing and emerging data standards to ensure high-quality, usable, and interoperable data, while also implementing robust cybersecurity and access safeguards consistent with Federal standards. For long-term preservation, the bill mandates the storage of collected samples, with ownership remaining with the program but physical housing potentially at the Smithsonian Institution or the Department of Agriculture. Before collection, species are evaluated to identify those needing long-term storage and high-priority species, with strict protocols for minimizing the risk of collecting or storing disease vectors or pathogens. Importantly, all physical samples collected under the program cannot be transferred, exported, or loaned outside the United States, and all storage, curation, and laboratory processing must occur domestically. The Secretary is required to select an initial 5 National Park units for the program within 180 days, expanding to an additional 20 units within two years, based on criteria such as biological landscape and research value. The bill also outlines an implementation plan, including potential expansion to other federally managed lands and facilities, and pathways for U.S.-based entities (not controlled by foreign entities of concern) to access data for artificial intelligence model development. Significant appropriations are authorized for the program from fiscal years 2027 through 2031, allocated across various agencies for program oversight, genome sequencing, sample storage, and data storage, with the program's authority terminating after 10 years.