This bill, known as the "WORK to Save Lives Act," directs the Secretary of Labor to issue guidance and regulations aimed at increasing the availability and safe use of opioid overdose reversal medication in workplaces. Within 270 days of enactment, the Secretary, through OSHA, must provide non-mandatory guidance for private employers on acquiring and maintaining these medications and offering voluntary annual employee training on their usage. Concurrently, the bill mandates the issuance of mandatory regulations requiring all Federal agencies, including the Veterans Health Administration, to acquire and maintain opioid overdose reversal medication. These regulations also compel Federal agencies to offer voluntary annual training to their employees on how to use the medication effectively. This legislation establishes distinct requirements for private and federal sectors to address opioid overdose preparedness.