This legislation aims to amend the Education Amendments of 1972 to clarify federal nondiscrimination requirements applicable to education programs or activities receiving federal financial assistance. Its primary purpose is to establish that discrimination prohibited under Title IX is based on the biological reality of sex . The Act explicitly defines the term "sex" as an individual's biologically determined sex , meaning either male or female. It further specifies that "female" refers to an individual who naturally possesses, or would possess, the reproductive system that produces ova for fertilization, while "male" refers to an individual who naturally possesses, or would possess, the reproductive system that produces sperm for fertilization, accounting for anomalies or disruptions. These amendments will take effect on the date of enactment and will apply to education programs and activities that receive federal financial assistance on or after that date.