This legislation reauthorizes and significantly improves the national wildlife crossings program, ensuring its continued operation and expansion. It authorizes an appropriation of $100,000,000 annually from the Highway Trust Fund for each of fiscal years 2027 through 2031, with these funds remaining available until expended. A major change is the removal of the "pilot" designation from the program, establishing it as a permanent initiative. The bill also introduces a higher federal share of 90 percent for projects benefiting small, rural, or disadvantaged communities , with the Secretary having the discretion to increase this to 100 percent in cases of financial hardship. Furthermore, it allocates up to 0.5 percent of program funds for Tribal technical assistance , aiming to help Tribal entities access funding more efficiently and expeditiously. Another 0.5 percent of funds may be retained by the Secretary for grant administration , covering application review, obligation, and award management.