This legislation reauthorizes and significantly improves the existing wildlife crossings program by allocating $200,000,000 annually from the Highway Trust Fund for fiscal years 2027 through 2031, with funds available until expended. A key change is the removal of the "pilot" designation, making the wildlife crossings program a permanent initiative to enhance wildlife safety and connectivity. The bill introduces specific provisions to support Indian Tribes, establishing a 100 percent Federal share for their projects and dedicating up to 0.5 percent of program funds for Tribal technical assistance to help them access funding efficiently. This assistance includes application support and efforts to reduce the time between project selection and fund obligation. An additional 0.5 percent is allocated for the Secretary's grant administration, ensuring effective oversight and implementation of the program.