This legislation mandates the Department of Transportation (DOT) to significantly bolster its initiatives to combat human trafficking across all transportation modes. It requires the Secretary of Transportation to leverage existing research and conduct new studies to better understand and address human trafficking within transportation operations, with a comprehensive report to Congress due within three years. To improve coordination and effectiveness, the bill directs the DOT to establish central databases for tracking counter-trafficking efforts by transportation organizations and for monitoring due diligence and prevention laws related to forced labor in supply chains. It also requires the development of mode-specific counter-trafficking policies and a zero-tolerance policy template for transportation entities, all developed in consultation with key anti-trafficking initiatives. Furthermore, the Secretary must update the Department's multimodal counter-trafficking training and awareness campaign within one year, incorporating survivor-informed and trauma-informed best practices and addressing technological barriers. This includes creating various educational materials like pocket cards, multilingual resources, and standardized checklists for identifying trafficking indicators, alongside an ongoing public awareness campaign targeting vulnerable routes and major events. A crucial component of the bill is the establishment of a new grant program, authorizing $10,000,000 annually from fiscal years 2027 through 2031, to support human trafficking awareness, education, and prevention efforts by multimodal transportation stakeholders. These grants will prioritize regions with a higher prevalence of human trafficking and will be distributed to eligible entities such as transit operators, airport authorities, and non-profit organizations.