This bill amends the National Trails System Act to officially designate the historic Route 66 as a National Historic Trail . This designation encompasses all alignments of U.S. Highway 66 that existed between 1926 and 1985, covering an approximate 2,400-mile route from Chicago, Illinois, to Santa Monica, California. The Secretary of the Interior will administer the trail, emphasizing respect for its unique and idiosyncratic nature , and must conduct active consultation with affected Indian Tribes for activities with substantial direct impacts. Crucially, the bill imposes significant restrictions on land acquisition, prohibiting the use of eminent domain or condemnation and limiting acquisitions to a quarter-mile average on either side of the trail, only with owner consent outside federal areas. The designation explicitly states that it does not create buffer zones, nor does it impede energy development, production, transportation, or transmission. Furthermore, it clarifies that the trail is not considered "lands in the National Park System" for certain purposes and does not introduce new federal permitting requirements or alter existing authorities for granting easements or rights-of-way.