The "Protect and Serve Act of 2025" amends federal law to create new criminal offenses specifically targeting law enforcement officers. It establishes penalties for individuals who knowingly assault a law enforcement officer , causing serious bodily injury or attempting to do so. Offenders could face imprisonment for up to 10 years and a fine. If the offense results in death, or includes kidnapping or an attempt to kill, the penalty increases to any term of years or life imprisonment, along with a fine. Federal jurisdiction for these crimes is established through various interstate commerce connections, such as the travel of the defendant or victim across state lines, the use of interstate channels, or if the victim is a federal officer. A key provision requires the Attorney General to certify federal prosecution, ensuring it is necessary due to state jurisdiction limitations, state request, unvindicated federal interest, or public interest to secure substantial justice.