This bill significantly strengthens the enforcement capabilities of the Attorney General under the Animal Welfare Act. It empowers the Attorney General to initiate civil actions in federal courts against violators, seeking remedies such as temporary restraining orders, injunctions, license revocations, and civil penalties up to $10,000 per violation per day. A crucial new authority allows for injunctions specifically including the removal or relocation of animals from harmful conditions. The legislation further enables the seizure and forfeiture of any animal involved in violations of the Act or its regulations. Responsible parties can be charged fees for the care of these seized animals, and funds from civil penalties or fines may be used to cover temporary animal care costs. The bill also clarifies that enforcement extends to all rules, standards, and regulations promulgated under the Animal Welfare Act. To ensure coordinated efforts, the bill mandates a memorandum of understanding between the Secretary of Agriculture and the Attorney General within 180 days. This agreement will facilitate information sharing, especially concerning repeat violators impacting animal health. These expanded powers for the Attorney General operate concurrently with, and do not limit, the existing authority of the Secretary of Agriculture.