This bill, titled the "Defend Girls Athletics Act," establishes new requirements for elementary schools, secondary schools, and institutions of higher education regarding participation in women's sports. It mandates that these educational entities must ensure "biological fairness" in women's sports as a prerequisite for receiving federal financial assistance. Specifically, the legislation requires compliance with Executive Order 14201 , which pertains to "keeping men out of women's sports." Local educational agencies must annually certify their adherence to these requirements to their respective state educational agencies, which then report compliance status to the Secretary of Education. For both K-12 schools and higher education institutions, failure to comply with these provisions will result in significant penalties. Non-compliant entities will be required to return any unobligated federal funds received and will become ineligible for future federal funding or participation in federal programs, including student loan programs, until compliance is achieved.