This bill mandates that the United States Military Academy, Naval Academy, and Air Force Academy prohibit individuals whose sex is male from participating in athletic programs or activities designated for women or girls. The Secretary of Defense is tasked with ensuring compliance with this prohibition across all three service academies. A key provision clarifies that males may still train or practice with female-designated teams, provided that no female is deprived of a roster spot, opportunity to participate, scholarship, or any other benefit associated with the athletic program. The bill broadly defines "athletic programs and activities" to include all related programs and activities. Furthermore, it explicitly defines "sex" as a person's reproductive biology and genetics at birth for the purposes of this legislation.