The "Habitat Connectivity on Working Lands Act of 2025" aims to significantly improve wildlife habitat connectivity and wildlife migration corridors , with a specific focus on big game species such as deer, elk, and moose. It achieves this by amending the Food Security Act of 1985, defining habitat connectivity as the degree to which landscapes facilitate native species movement among seasonal habitats. The legislation integrates these conservation goals into existing federal agricultural programs. The bill expands the Regional Conservation Partnership Program (RCPP) to include the restoration and enhancement of wildlife habitat connectivity and migration corridors as critical conservation areas. It also allows for cost-share payments under the Environmental Quality Incentives Program (EQIP) and the Conservation Stewardship Program (CSP) for practices on ecologically significant grasslands enrolled in the Conservation Reserve Program (CRP). Importantly, for CSP, these new provisions explicitly state that emergency grazing and haying access on CRP grasslands will be preserved, and the maximum rental payment for CRP is increased from $50,000 to $125,000. To further support these efforts, the legislation mandates the incorporation of nonstructural methods for livestock distribution, such as virtual fencing, into conservation practice standards. The Secretary of Agriculture must ensure adequate technical assistance is available for implementing these innovative methods and other habitat connectivity practices. Furthermore, the bill designates virtual fencing as a high-priority research and extension area , encouraging studies on its adoption barriers and its effects on natural resources, including crucial winter ranges for big game species.