The "Protecting Horses from Soring Act of 2025" aims to significantly strengthen the Horse Protection Act by introducing new measures to combat the practice of horse soring. It mandates the establishment of a new, centralized Horse Industry Organization (HIO) within 180 days of enactment, replacing the previous fragmented system. This HIO will be responsible for overseeing inspections and ensuring compliance with anti-soring regulations. A key provision is the definition and implementation of "objective inspections," which must be conducted by veterinarians or technicians using science-based protocols like swabbing and blood testing, validated through peer review and scientific acceptance. The HIO will be governed by a board with specific appointments, including representatives from Tennessee and Kentucky agriculture and the Tennessee Walking Horse industry. Its responsibilities include formal affiliation with all horse events, appointing inspectors, and contracting equine veterinary experts. Crucially, the HIO will develop licensing requirements for inspectors, mandating that these individuals and their immediate families be free from conflicts of interest with the walking horse industry. Under the amended Act, horses determined to be sore by an objective inspection will face mandatory disqualification periods, starting with 30 days for a first offense and 90 days for subsequent violations. The Secretary of Agriculture will certify this new HIO to train and license inspectors, while simultaneously revoking certifications for any other existing horse industry organizations. The bill requires the Secretary to issue comprehensive regulations within 180 days to fully implement these amendments.