This bill proposes to prohibit entities from receiving Federal funds if they allow individuals to access or use a single-sex facility that does not correspond to their biological sex . The legislation defines "biological sex" based on the reproductive system's capacity to produce eggs (female) or sperm (male), and specifies that "single-sex facilities" include restrooms, locker rooms, and changing rooms. This prohibition applies broadly to various entities, including private organizations, non-Federal government agencies, and State, Tribal, or local governments, aiming to enforce sex-segregated spaces based on these biological definitions. However, the bill provides specific, limited exceptions to this funding restriction. These exceptions allow emergency medical personnel to access such facilities during a medical emergency, and permit law enforcement officers to do so when in active pursuit of a suspect or as part of an active investigation. These are the only circumstances under which an individual may access a facility not corresponding to their biological sex without jeopardizing an entity's federal funding.