This bill prohibits individuals from accessing or using single-sex facilities on Federal property unless those facilities correspond to their biological sex. These facilities include restrooms, locker rooms, and changing rooms located on any property owned, leased, or occupied by the United States government or its instrumentalities. The legislation defines biological sex as the biological determination of male or female, providing specific criteria based on reproductive systems. A female is defined by a reproductive system that produces eggs, and a male by one that produces sperm, accounting for developmental or genetic anomalies. Exceptions to this prohibition are made for emergency medical personnel responding to incidents and for law enforcement officers during active pursuit or investigations.