Department of Energy Biological Innovation Opportunities Act or the Department of Energy BIO Act This bill requires the Advanced Research Projects Agency-Energy (ARPA-E) within the Department of Energy to select and establish up to four bioenergy research centers. The centers must conduct research to inform the production of biofuels and to facilitate the translation of the research results to industry. ARPA-E may select existing bioenergy research centers and provide support for them under this bill.